Pharmaceutical pictograms are pictorial interventions that aid in patient counselling and education, which have been proven to improve medication adherence and understanding of medication information. This intervention could benefit individuals with chronic medical conditions, which often deal with polypharmacy, possibly leading to nonadherence to medication behaviours. The objective of this research is to evaluate the USPmodified pharmaceutical pictograms comprehension among individuals with chronic medical conditions in Klang Valley, Malaysia, thus establishing a feasible set of pharmaceutical pictograms among the Malaysian population. This cross-sectional study was conducted through an online questionnaire via a convenience sampling method from April to July 2023. Twenty-three modified USP pharmaceutical pictograms were used in this study. The inclusion criteria of participants were individuals with chronic medical conditions of age 18 and above, any gender, or ethnicity, living in Klang Valley, Malaysia, diagnosed with any chronic medical conditions, and receiving medicines at least for last one year. The data collected included the socio-demographics of participants, transparency, and translucency of each pictogram. Pearson’s correlation test and Spearman’s rank correlation test were performed to analyse the data. A total of 115 participants were included in this study after excluding sixteen participants who did not meet the inclusion criteria. Overall, 16 (69.5%) out of 23 pictograms met both the transparency and translucency criteria, and hence were considered valid. All the pictograms (100%; 10 out of 10) depicting the dose and frequency of administration were deemed valid. On average, the majority of participants with chronic medical conditions were able to achieve comprehensibility of at least 66% correct responses on depicting the pictograms. Pictograms that did not meet the validity requirement need further modification. The study recommends that future studies should be conducted on a wider population and should focus on the utilization of pharmaceutical pictograms into real practice. Keywords: Evaluation, Malaysia, Pharmaceutical Pictograms, Chronic Medical Conditions, Medications.