Nanofibers have become a prominent research focus in wound dressing applications due to their exceptional material properties. The drug-loaded nanofibers can effectively eliminate the bacterial growth in the wound site. This study presents the formulation and fabrication of chitosan-gelatin (CS-GEL) nanofibers incorporated with curcumin and copper/zinc-based metal-organic framework (Cu/Zn-MOF) using the electrospinning method. The Cu/Zn-MOF was analysed using X-ray diffraction (XRD). The CS-GEL nanofibers loaded with curcumin and copper/zinc MOF were characterized with ATR-FTIR spectroscopy and scanning electron microscopy (SEM). The in-vitro antioxidant, anti-inflammatory, cytotoxicity, and antibacterial properties were also evaluated. The optimal conditions for smooth nanofiber production were identified as a polymer concentration of 3% (w/v) chitosan and 45% (w/v) gelatin, with a 30:70 chitosan-to-gelatin ratio. The optimized electrospinning parameters included an applied voltage of 18 kV, a flow rate of 0.8 mL/h, and a working distance of 15 cm. The optimized concentration of curcumin-Cu/Zn-MOF/CS-GEL nanofibers showed no toxic effects on HaCaT cells in 5% (w/v) of curcumin. Additionally, the 5CUR/10CuZn-MOF/CS-GEL nanofiber sample showed the highest percentage of DPPH radical scavenging (83.70%) for antioxidant activity. The highest percentage inhibition for the formulation of curcumin and MOF was 83.10%. The incorporation of curcumin and Cu/Zn-MOF effectively inhibited the growth of S. aureus and P. aeruginosa in antibacterial assays. The 5CUR/10CuZn-MOF/CS-GEL nanofiber sample was the most effective formulation against S. aureus and P. aeruginosa for antibacterial test. The 5CUR/10CuZn-MOF/CS-GEL nanofiber sample also showed the anti-biofilm effect against S. aureus. The 5CUR/1CuZn-MOF/CS-GEL, 5CUR/5CuZn-MOF/CS-GEL and 5CUR/10CuZn-MOF/CS-GEL nanofiber samples demonstrated potential applications for wound healing, as they were non-toxic to cells and exhibited antibacterial activity, which showed wound healing rate of 95.30%, 99.30% and 96.96%, respectively. The potential application of advanced wound dressings and nanotechnology lies in their ability to enhance healing rates and improve treatment outcomes.