This research aims to study the acceptance of the IJNPulse mobile application among IJN staff using the Unified Theory of Acceptance and Use of Technology (UTAUT) framework. It focuses on understanding the adoption and usability of this novel staff mobile application, exploring staff attitudes and experiences with its use, and identifying key factors that influence their intention to adopt the application. A cross-sectional survey design was employed to collect data from a diverse sample, incorporating a defined research paradigm, specific sampling techniques, and systematic data collection and analysis methods. The findings reveal that performance expectancy, social influence, and facilitating conditions positively influence IJN staff’s behavioral intentions and actual use of the IJNPulse application. However, effort expectancy was not found to significantly affect adoption or user behavior. These results provide valuable insights into the factors that shape staff acceptance of mobile health applications in a healthcare setting.