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ANTIDIABETIC ACTIVITY OF CATALPOL ON HIGH FAT DIET AND STREPTOZOTOCIN INDUCED TYPE-2 DIABETES MICE BY IMPROVING INSULIN SENSITIVITY AND MITOCHONDRIAL FUNCTION

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dc.contributor.authorYAP KAH HENG
dc.date.accessioned2024-07-12T08:40:02Z
dc.date.available2024-07-12T08:40:02Z
dc.date.issued2023
dc.description.abstractThere is an urgent need in search of an insulin-sensitising agent exhibiting good safety, efficacy and tolerability profile for the prevention and treatment of type-2 diabetes mellitus. Catalpol, an iridoid glucoside, is the main bioactive component of Rehmannia glutinosa radix. Catalpol is reported to possess anti-oxidant, neuro-protective and glucose-lowering effects. The work described in this study aimed to examine the effects of catalpol in improving glycaemic control as well as insulin sensitivity in both skeletal muscles and liver of type-2 diabetes mellitus mice. Male C57BL/6N mice aged 5-6 weeks were placed on a high fat diet (60% kcal) for two weeks prior to streptozotocin (50 mg/kg, i.p.) administration for three consecutive days. Group fed with normal chow diet served as the normal control. Four groups of diabetic animals were administered orally with saline, catalpol (100 mg/kg), catalpol (200 mg/kg) or metformin (200 mg/kg) once per day for four consecutive weeks. Treatment of catalpol (200 mg/kg) significantly lowered fasting plasma glucose compared to diabetic control (p < 0.01), along with attenuation of insulin resistance by reducing HOMA-IR index. Catalpol improved insulin sensitivity in skeletal muscles through activating AMPK/SIRT1/PGC-1a pathway. The downstream effect was observed in improved mitochondrial respiration. Administration of catalpol increased the insulin sensitivity in liver by up-regulating PPAR-g and suppressing the expression of gluconeogenic enzymes. Besides, catalpol treatment improved lipid metabolism by regulating plasma adiponectin, plasma triglycerides and liver triglycerides levels. Collectively, catalpol (200 mg/kg) exerted anti-diabetic effects by improving glycaemic control, insulin sensitivity and mitochondrial function in skeletal muscles. Besides, catalpol improved insulin sensitivity in liver and lipid metabolism.
dc.identifier.urihttps://hdl.handle.net/20.500.14377/36539
dc.language.isoen
dc.publisherInternational Medical University
dc.subjectDiabetes Mellitus, Type 2
dc.subjectInsulin
dc.subjectGlycemic Control
dc.subjectInsulin Resistance
dc.subjectDiet, High-Fat
dc.subjectLipid Metabolism
dc.titleANTIDIABETIC ACTIVITY OF CATALPOL ON HIGH FAT DIET AND STREPTOZOTOCIN INDUCED TYPE-2 DIABETES MICE BY IMPROVING INSULIN SENSITIVITY AND MITOCHONDRIAL FUNCTION
dc.typeThesis
dspace.entity.typePublication
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
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