Publication: EXPLORING THE POTENTIAL FOR QUERCETIN- POTENTIATION OF THE CARDIOVASCULAR EFFECTS OF METFORMIN, A BIGUANIDE ANTIDIABETIC DRUG
| dc.contributor.author | JESTIN CHELLIAN | |
| dc.date.accessioned | 2023-10-06T15:40:26Z | |
| dc.date.available | 2023-10-06T15:40:26Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Endothelial dysfunction is proven to be the fundamental cause in the pathogenesis of cardiovascular diseases and diabetes. The present investigation aims to examine the effects of quercetin, metformin as well as their combination on endothelial dysfunction in T1DM and T2DM rats. Male Sprague Dawley rats were used in this study. T1DM was induced by an intraperitoneal (i.p.) injection of streptozotocin (STZ, 65 mg/kg body weight (b.w.)(1). The diabetic rats in both groups were orally fed with quercetin (10 mg/kg b.w.), metformin (180 mg/kg b.w.) as well as their combination (quercetin (10 mg/kg b.w.) and metformin (180 mg/kg b.w.)(2). The treatment period was 30 days. At the end of the study, blood was collected, and the rats were sacrificed by cervical dislocation followed by excision of liver, aorta, and pancreas. The plasma biochemical parameters such as glucose, high-density lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol (TC), triglycerides (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatinine were estimated. All these levels except HDL were found to be elevated from normal range in both T1DM and T2DM rats. It was observed that the test compounds reversed these elevated levels. Histological studies on liver, aortae, pancreas and kidneys revealed that diabetic rats do exhibit abnormal changes in the architecture of the organs, while in diabetic rats treated with test compounds, the changes in architecture of the organs is minimal compared to normal control rats. The quercetin plus metformin combination was found to be the most potent followed by individual metformin and quercetin in sequence. Furthermore, in diabetic rats, the relaxation effect of acetylcholine (ACh) and sodium nitroprusside (SNP) were diminished compared to normal control rats; while, in diabetic rats treated with test compounds, the acetylcholine (ACh) and sodium nitroprusside (SNP) induced relaxation effects were higher than those in diabetic control group. The phenylephrine (PE) induced contractile effect in diabetic rats treated with test compounds was elevated compared to the diabetic control. Immunohistochemical studies revealed that expression levels of eNOS and SIRT1 were found to be higher in quercetin plus metformin treated group followed by metformin and quercetin individual treatment. In rats treated with test compounds, the levels of 3-NT, VCAM-1 and CD31 were lower compared to the diabetic control. Overall, it can be concluded that quercetin potentiated the effect of metformin in improving the endothelial function in both T1DM and T2DM diabetic rats. In all estimations, the quercetin plus metformin combination was found to be the most potent followed by individual metformin and quercetin in sequence. | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.14377/32313 | |
| dc.language.iso | en | en_US |
| dc.publisher | International Medical University | en_US |
| dc.subject | Quercetin | en_US |
| dc.subject | Metformin | en_US |
| dc.subject | Diabetes Mellitus, Type 2 | en_US |
| dc.subject | Rats | en_US |
| dc.title | EXPLORING THE POTENTIAL FOR QUERCETIN- POTENTIATION OF THE CARDIOVASCULAR EFFECTS OF METFORMIN, A BIGUANIDE ANTIDIABETIC DRUG | en_US |
| dc.type | Thesis | |
| dspace.entity.type | Publication |