Publication: ANTICANCER EFFECTS OF SAIKOSAPONIN A AND SAIKOSAPONIN D IN BREAST CANCER CELL LINES
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
International Medical University
Abstract
Breast cancer cells often respond poorly to treatment because of their different subtypes such as low expression of the oestrogen, progesterone and receptor tyrosine-protein kinase erbB-2. Hence, there is a need to identify new molecules for the treatment. Radix Bupleurix contains glucosides, a class of oleanane derivatives called saikosaponins that have been widely used in Traditional Chinese Medicine for over a millennium. There are two main active components in Radix Bupleurix, Saikosaponin A (SSA) and Saikosaponin D (SSD), known to have a wide range of pharmacological actions. This study identifies the anti-cancer effect of SSA and SSD on breast cancer cell lines, MDA-MB-468, MCF7 and T47D. Our findings suggested that SSA and SSD can inhibit the growth of the breast cancer cell line in a dose-response-dependent manner. The IC50 for SSA and SSD ranges between 4.48 μM to 6.97 μM. SSA and SSD can inhibit cell migration and colony formation. Scratch assay results showed a significant area of closure compared to control (p < 0.05) after 120 hours. Furthermore, a combination assay showed no synergistic effect between SSA and SSD. In conclusion, these results suggest that SSA and SSD could be potential molecules for the treatment of breast cancer but further study is required to understand the pathway involved in the anti-cancer effect.
Description
Keywords
Breast Neoplasms, Estrogens, Progesterone, Glucosides, Medicine, Chinese Traditional, Cell Line