Publication: IMMUNOMODULATORY EFFECTS OF CHINESE CATERPILLAR MUSHROOM, OPHIOCORDYCEPS SINENSIS CULTIVAR OCS02® (ASCOMYCETES) IN SENSITISED NCTC2544 HUMAN KERATINOCYTE CELL LINE
Date
2023
Authors
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Publisher
International Medical University
Abstract
Contact dermatitis (CD) is a common skin condition characterised by inflammation caused
by exposure to allergens. In Malaysia, it accounted for a significant portion of skin diseases, with
irritant contact dermatitis being the most prevalent type. Ophiocordyceps sinensis, a medicinal
mushroom, has been extensively studied in various disease models and has multiple therapeutic
properties in treating hyperlipidemia, arrhythmias and other disorders The objective of this study
was to investigate the immunomodulatory effects of O. sinensis cultivar OCS02® on sensitized
NCTC 2544 keratinocytes. An in vitro assay was conducted to evaluate the cytotoxicity, expression
of cytokines, ROS, and lipid peroxidation in sensitised NCTC 2544 keratinocytes exposed to
OCS02® cold water extract (CWE).
The results revealed OCS02®CWE were less cytotoxic to keratinocytes than
paraphenylenediamine (PPD) with half maximal inhibitory concentration (IC50) 650 μg/mL and
17.7 μg/mL respectively. OCS02® CWE (at 250 μg/mL) significant down-regulated the
expression of reactive oxygen species, peroxidation of membrane lipid bilayer, interleukin (IL)-18
and IL-6 cytokines in PPD-sensitised keratinocytes (p<0.05). This study reveals that O. sinensis
and its extracts possess anti-inflammatory and cytoprotective properties, suggesting their potential
utility in managing inflammatory conditions. Furthermore, the immunomodulatory effects
observed in cytokine production indicate their promising role in alleviating diseases characterized
by chronic inflammation and immune dysfunction. In conclusion, OCS02® CWE may have
immunomodulatory application for the treatment of CD.
Description
Keywords
Dermatitis, Contact, Inflammation, Allergens, Skin Diseases, Keratinocytes, Cell Line