Publication: EVALUATION OF ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF DOANI SIDR HONEY AND MADECASSOSIDE AGAINST PROPIONIBACTERIUM ACNES IN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCs)
| dc.contributor.author | HANA AL-BAGHAOI ABDULLAH | |
| dc.date.accessioned | 2023-10-06T15:32:08Z | |
| dc.date.available | 2023-10-06T15:32:08Z | |
| dc.date.issued | 2015-10 | |
| dc.description.abstract | Propionibacterium acnes (P. acnes), plays a key role in acne pathogenesis by inducing the innate immune system cells to produce certain pro-inflammatory mediators which contribute to the formation of inflammatory acne lesion. The present study was conducted to evaluate the potential of Doani sidr honey and honey polysaccharide rich fraction and madecassoside for antimicrobial and anti-inflammatory activity against P. acnes. The broth dilution method was used to assess the antibacterial activity and the ultrastructural changes in cell morphology before and after exposure to tested samples were studied using transmission electron microscopy (TEM). The tested samples were further investigated for the suppression of pro-inflammatory cytokines tumour necrosis factor (TNF-α) and interleukin 8 (IL-8) by testing the supernatants in the co-culture of the human peripheral blood mononuclear cells (PBMCs) and heat killed P. acnes using enzyme immunoassay kits (ELISA). The statistical analysis was done using the Graph Pad Prism 5 program. The Doani sidr honey and its polysaccharide rich fraction were able to inhibit the growth of P. acnes with a noteworthy minimum inhibitory concentration (MIC) value of 18%(w/v) and 29 %(w/v) respectively and also the release of TNF-α and IL-8 pro-inflammatory cytokines by suppressing them by more than 90%. TEM micrographs revealed the lethal effects of Doani sidr honey against P. acnes .However, no significant inhibition of the P. acnes growth and the secretion of TNF-α and IL-8 was detected for madecassoside at its highest tested concentration. Our results suggested that Doani sidr honey possess both antimicrobial and anti-inflammatory effects against P. acnes and could possibly be used as therapeutic agents for acne. Furthermore, polysaccharide fraction derived from Doani sidr honey showed potent inhibitory effect toward P. acnes. Hence, we hypothesise the fraction prepared from Doani sidr honey might contribute to honey activity. Therefore, this fraction needs to be further explored and characterised to find the main contributing phytochemicals and the antibacterial and anti-inflammatory properties in this fraction. | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.14377/32213 | |
| dc.language.iso | en | en_US |
| dc.publisher | International Medical University | en_US |
| dc.subject | Propionibacterium acnes | en_US |
| dc.subject | Honey | en_US |
| dc.subject | Anti-Infective Agents | en_US |
| dc.subject | Anti-Inflammatory Agents | en_US |
| dc.subject | In Vitro Techniques | en_US |
| dc.title | EVALUATION OF ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF DOANI SIDR HONEY AND MADECASSOSIDE AGAINST PROPIONIBACTERIUM ACNES IN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCs) | en_US |
| dc.type | Thesis | |
| dspace.entity.type | Publication |