Publication: PHYSICOCHEMICAL PROPERTIES, CYTOTOXICITY AND ANTIBACTERIAL ACTIVITY OF ELECTROSPUN POLYCAPROLACTONE NANOFIBERS LOADED WITH CURCUMIN, ZINC OXIDE AND TITANIUM DIOXIDE
| dc.contributor.author | LEE YEONG HAN | |
| dc.date.accessioned | 2024-07-05T02:39:01Z | |
| dc.date.available | 2024-07-05T02:39:01Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Nanofibers for wound dressing application has become the research hotspot due to their excellent properties to make wound dressing. The nanofibers can be loaded with the drugs to kill or inhibit the bacteria on wound area. This study demonstrated the preparation and formulation of polycaprolactone (PCL) nanofibers loaded with curcumin, titanium dioxide and zinc oxide using electrospinning method. Optimisation of the PCL nanofibers for each parameter of electrospinning was done before preparing curcumin-titanium dioxide/zinc oxide nanofiber. Scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, and differential scanning calorimetry (DSC) were then used to characterise the nanofibers. Surface properties measurement, degradation analysis, in-vitro release of drug test, in-vitro cytotoxicity study, and antibacterial activity were evaluated on nanofibers. The optimised parameters to produce smooth nanofibers were found to be 12 wt% polymer concentration, a 6:4 mixture of chloroform and dimethylformamide at room temperature, 15 kV applied voltage, 0.5 mL/h flow rate, and a 20 cm working distance. The addition of curcumin, titanium dioxide nanoparticles, and zinc oxide nanoparticles into the nanofibers can increased the diameter and hydrophobicity of the PCL nanofibers but decreased its crystallisation and melting temperature. The degradation test showed that the PCL nanofibers were able to degrade completely within 14 days with the presence of lipase. In drug release study, the nanofiber exhibited a burst release of curcumin at the beginning of time, then sustained release. In cytotoxicity study, 1 and 3% w/w of curcumin in nanofibers do not show cytotoxic effect to HaCaT cells. The addition of curcumin-titanium dioxide/zinc oxide can suppress the activity of Staphylococcus aureus and Pseudomonas aeruginosa in antimicrobial tests. The curcumin-titanium dioxide and zinc oxide nanofibers with 1 and 3% w/w of curcumin show potential application in wound healing, as they do not harm the cells and able to inhibit the activity of bacteria, which will be useful to protect the wound. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14377/36529 | |
| dc.language.iso | en | |
| dc.publisher | IMU University | |
| dc.subject | Nanofibers | |
| dc.subject | Bandages | |
| dc.subject | Wounds and Injuries | |
| dc.subject | Microscopy, Electron, Scanning | |
| dc.title | PHYSICOCHEMICAL PROPERTIES, CYTOTOXICITY AND ANTIBACTERIAL ACTIVITY OF ELECTROSPUN POLYCAPROLACTONE NANOFIBERS LOADED WITH CURCUMIN, ZINC OXIDE AND TITANIUM DIOXIDE | |
| dc.type | Thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# |