Publication: THE INFLUENCE OF CONDITIONED MEDIA FROM PANCREATIC CANCER CELL LINES (ASPC1, PANC10.05, SW1990 AND BXPC3) ON THE VIABILITY AND PROLIFERATION OF PANCREATIC STELLATE CELLS
Date
2023
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Publisher
International Medical University
Abstract
Pancreatic ductal adenocarcinoma (PDAC) had a bad prognosis where the patient often has zero
or easily dismissed symptoms. Pancreatic cancer is often detected in the late-stage and
pancreatic cancer patients’ survival rate is greatly reduced after 5 years. The pancreatic stellate
cell (PSC) takes up a small part of the pancreas. Pancreatic cancer cells (PCC) and PSC were
found to co-influence each other when cultured together. PSC is quiescent in nature and is
activated by transforming growth factor-beta (TGF- β), sonic hedgehog (Shh), and platelet-
derived growth factor (PDGF) and these factors are found in PCC. This study is aimed to
investigate how PCC and PSC co-influence each other. PSCs were treated with conditioned
media of four different PCC cell lines (AsPC1, PANC10.05, SW1990 and BxPC3) and the
viability of PSCs was measured. These four cell lines represent pancreatic cancer at various
stages. Our findings showed that PCC conditioned media (CM) at different concentrations does
affect PSC viability. PSC showed significantly reduced viability when treated with 30% of
SW1990 and AsPC-1 CM at 24-hour intervals. Meanwhile, at 48-hour intervals, PSC showed
significantly decreased viability in BxPC-3 and SW1990 CM. In conclusion, PCC of different
cancer stages does affect the viability of PSCs.
Description
Keywords
Pancreatic Ducts, Pancreatic Stellate Cells, Pancreatic Neoplasms, Intercellular Signaling Peptides and Proteins, Cell Line