Publication: CYTOTOXIC AND APOPTOGENIC EFFECTS OF SYZYGIUM AQUEUM EXTRACTS ON PANCREATIC CANCER CELL LINE
| dc.contributor.author | JOSHUA LOW JUN YI | |
| dc.date.accessioned | 2026-07-25T02:21:22Z | |
| dc.date.available | 2026-07-25T02:21:22Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Pancreatic cancer (PC) remains a highly lethal malignancy with limited therapeutic options, highlighting the need for alternatives with selective anticancer activity. Syzygium aqueum, a phytochemical-rich tropical plant, exhibits diverse bioactivities, yet its anticancer effects against PC have not been investigated. This study assessed the cytotoxic, antiproliferative, and apoptogenic effects of ten S. aqueum leaf and stem extracts, including hexane, chloroform, ethyl acetate, methanol, and aqueous extracts, on BxPC-3 cells, with selectivity evaluated in non-cancerous 3T3 fibroblasts. Cytotoxicity was assessed by MTT assays at 6.25–200 μg/ml over 24, 48, and 72 hours. Antiproliferative and apoptotic effects were assessed by doubling time analysis and Hoechst 33342 staining, respectively, with all experiments performed in triplicate. At 72 hours, leaf ethyl acetate (LE), stem aqueous (SA), leaf chloroform (LC), and stem ethyl acetate (SE) showed pronounced cytotoxicity, with IC50 values of 49.3, 50.08, 61.23, and 71.80 μg/ml, respectively, and the 72-hour time point was selected for downstream analyses due to consistent IC50 values. Stem hexane (SH) displayed the highest selectivity index exceeding 3, indicating predominantly cancer-specific cytostatic effects. Doubling time analysis demonstrated significant (p < 0.05), dose-dependent proliferation delays for all extracts except leaf aqueous (LA) and LE, whereas Hoechst staining revealed significant increases (p < 0.05) in apoptotic cells across all treatments except SH, which showed minimal apoptosis consistent with its cytostatic profile. Collectively, these findings demonstrate that S. aqueum extracts exhibit promising anticancer activity against PC cells through combined cytotoxic, cytostatic, and pro-apoptotic effects, supporting further mechanistic investigation and therapeutic development. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14377/37776 | |
| dc.language.iso | en | |
| dc.publisher | IMU University | |
| dc.subject | Pancreatic Neoplasms | |
| dc.subject | Apoptosis | |
| dc.subject | Plant Extracts | |
| dc.subject | Syzygium | |
| dc.subject | Cell Line, Tumor | |
| dc.subject | Antineoplastic Agents | |
| dc.title | CYTOTOXIC AND APOPTOGENIC EFFECTS OF SYZYGIUM AQUEUM EXTRACTS ON PANCREATIC CANCER CELL LINE | |
| dc.type | Thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# |