Publication: COMPARATIVE ANALYSIS OF COMMERCIAL Ω-3 FISH OILS IN CORRELATION WITH THEIR COMPOSITION AND NEUROPROTECTIVE PROPERTIES: A CHEMOMETRIC STUDY USING SPECTROSCOPIC AND BIOASSAY METHODS
| dc.contributor.author | MARCELINA ALOYCE MTALO | |
| dc.date.accessioned | 2025-11-12T01:55:18Z | |
| dc.date.available | 2025-11-12T01:55:18Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Omega-3 fatty acids (ω-3 FAs) includes eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have attracted considerable interest for their likely health advantages. Many different benefits have been claimed for ω-3 fish oils, including preventing cardiovascular diseases (CVD), reducing cognitive decline, and helping improve and manage inflammatory diseases such as asthma, neurodegeneration, and arthritis. This study investigates the chemical profiles and heterogeneity of various commercial omega-3 fish oil products by chemometric cluster analysis of spectroscopic data, with specific focus on correlating these findings with their neuroprotective activity in microglial cells. Acquiring spectra was enabled using spectrophotometers: FTIR (Shimadzu) and 1H-NMR-Nanalysis equipped with MestReNova software operating at 61 MHz. A bioassay experiment was performed on the BV2 Murine microglia cell line, where Nitrite (NO) production was measured through the Griess assay. The NMR spectra were analyzed through chemometric analysis by principal component analysis (PCA), identifying patterns for the classification of the samples by determining similarities among them based on potency and establishing the relationship between the spectra data and NO production of the samples. The assessment of fish oils using spectroscopy techniques (FTIR and 1H-NMR) revealed the presence of ω-3 FAs compounds as their spectra fitted the DHA and EPA reference standards. The concentrations of the fish oils corresponded relatively inversely with the NO production measured through the Griess assay. DHA and EPA significantly reduced NO production on BV2 microglial cells, revealing the neuroprotective properties. The concentration range of DHA and EPA determined in samples was meaningful to exhibit the corresponding neuroprotective properties of the compounds. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14377/37243 | |
| dc.language.iso | en | |
| dc.publisher | IMU University | |
| dc.subject | Fatty Acids, Omega-3 | |
| dc.subject | Eicosapentaenoic Acid | |
| dc.subject | Docosahexaenoic Acids | |
| dc.subject | Fish Oils | |
| dc.subject | Spectrum Analysis | |
| dc.subject | Neuroprotection | |
| dc.subject | Biological Assay | |
| dc.title | COMPARATIVE ANALYSIS OF COMMERCIAL Ω-3 FISH OILS IN CORRELATION WITH THEIR COMPOSITION AND NEUROPROTECTIVE PROPERTIES: A CHEMOMETRIC STUDY USING SPECTROSCOPIC AND BIOASSAY METHODS | |
| dc.type | Thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# |