Publication: COMPARING PROTEOMIC PROFILES OF PRIMARY PANCREATIC DUCTAL ADENOCARCINOMA CELLS (PDACs), PANCREATIC STELLATE CELLS (PSCs) AND THEIR CO-CULTURE
Date
2022
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Publisher
International Medical University
Abstract
Pancreatic ductal adenocarcinoma (PDA) has a complex tumour microenvironment (TME) that are made up of pancreatic ductal adenocarcinoma cells (PDACs) and pancreatic stellate cells (PSCs). PDACs and PSCs’ interaction allows pancreatic cancer cells to evade the immune system and enhance tumour progression. Hence, identifying and comparing the proteomic profile of PDACs, PSCs and their co-culture will determine which genes are involved in the progression of PDA. PANC10.05 cells (primary PDAC cell line), PSC (hPSC21-S/T) cells and PANC10.05/PSCs co-culture were cultured in 6-well plate for 3 days. Cells collected were lysed to extract the proteins within the cells. The extracted protein lysate then undergoes insolution digestion of proteins. The protein in the samples were analysed and quantified through LCMS/MS before proceeding to data analysis via DEBrowser.
The results showed that VIM, KRT8 and KRT18 were differentially expressed proteins (DEPs) detected when compared between PANC10.05, PSCs and their co-culture. In this study, monoculture of PANC10.05 and PSCs were able to express proteins without any interaction between PANC10.05 and PSCs. Hence, PANC10.05-PSCs co-culture is not required for the expression of proteins. The expression of DEPs in PANC10.05 and PSCs are associated in the progression, invasion and metastasis of PDA.
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Keywords
Adenocarcinoma, Pancreatic Stellate Cells, Pancreatic Neoplasms, Cell Line