Publication: EFFECTS ON B LYMPHOCYTE PROLIFERATION BY CONDITIONED MEDIA FROM PANCREATIC DUCTAL ADENOCARCINOMA AND PANCREATIC STELLATE CELLS AND CO-CULTURE OF MYELOID DERIVED SUPPRESSOR CELLS
Date
2021
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
International Medical University
Abstract
Pancreatic ductal adenocarcinoma cells (PDACs) and pancreatic stellate cells (PSC in a concerted effort protect the tumour microenvironment from immune cells attack. However, most studies have solely focused on their immunosuppressive effects onto T cells, hence, our effort is to study their immunosuppressive effects on B cells viability. Two PDAC cell lines (PANC 10.05 and SW1990) and/or PSC were cultured for 3 days in DMEM : DMEM:f12 (50:50) at 37 oC with 5% CO2 to obtain conditioned media (CM). Collected CM were used to differentiate isolated PBMCs into MDSCs. B cells were isolated from whole blood and treated with CM and on a separate event with isolated MDSCs for 48 hours before proceeding with CellTiter Glo Viability Assay.
The cell viability assay showed that CM from PDACs and/or PSCs co-culture are both able to promote B cell proliferation (p<0.05). Similarly, all CM generated MDSCs was also able to promote B cell proliferation but MDSCs untreated by CM is not able to promote B cell proliferation effectively (p < 0.05). These findings indicate that MDSCs’ pro-B cell proliferation activity requires activation by the conditioned media.
Description
Keywords
Adenocarcinoma, Cell Line, Pancreatic Stellate Cells, Pancreatic Ducts, B-Lymphocytes, Coculture Techniques