Publication: EFFECTS ON B LYMPHOCYTE PROLIFERATION BY CONDITIONED MEDIA FROM PANCREATIC DUCTAL ADENOCARCINOMA AND PANCREATIC STELLATE CELLS AND CO-CULTURE OF MYELOID DERIVED SUPPRESSOR CELLS
| dc.contributor.author | EVELYN PRIYA A/P PETER | |
| dc.date.accessioned | 2023-10-06T15:32:16Z | |
| dc.date.available | 2023-10-06T15:32:16Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Pancreatic ductal adenocarcinoma cells (PDACs) and pancreatic stellate cells (PSC in a concerted effort protect the tumour microenvironment from immune cells attack. However, most studies have solely focused on their immunosuppressive effects onto T cells, hence, our effort is to study their immunosuppressive effects on B cells viability. Two PDAC cell lines (PANC 10.05 and SW1990) and/or PSC were cultured for 3 days in DMEM : DMEM:f12 (50:50) at 37 oC with 5% CO2 to obtain conditioned media (CM). Collected CM were used to differentiate isolated PBMCs into MDSCs. B cells were isolated from whole blood and treated with CM and on a separate event with isolated MDSCs for 48 hours before proceeding with CellTiter Glo Viability Assay. The cell viability assay showed that CM from PDACs and/or PSCs co-culture are both able to promote B cell proliferation (p<0.05). Similarly, all CM generated MDSCs was also able to promote B cell proliferation but MDSCs untreated by CM is not able to promote B cell proliferation effectively (p < 0.05). These findings indicate that MDSCs’ pro-B cell proliferation activity requires activation by the conditioned media. | en_US |
| dc.identifier.uri | ${dspace.baseUrl}/xmlui/handle/1234.56789/2066 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14377/32227 | |
| dc.language.iso | en | en_US |
| dc.publisher | International Medical University | en_US |
| dc.subject | Adenocarcinoma | en_US |
| dc.subject | Cell Line | en_US |
| dc.subject | Pancreatic Stellate Cells | en_US |
| dc.subject | Pancreatic Ducts | en_US |
| dc.subject | B-Lymphocytes | en_US |
| dc.subject | Coculture Techniques | en_US |
| dc.title | EFFECTS ON B LYMPHOCYTE PROLIFERATION BY CONDITIONED MEDIA FROM PANCREATIC DUCTAL ADENOCARCINOMA AND PANCREATIC STELLATE CELLS AND CO-CULTURE OF MYELOID DERIVED SUPPRESSOR CELLS | en_US |
| dc.type | Thesis | |
| dspace.entity.type | Publication |