Publication: THE INFLUENCE OF GEOGRAPHICAL LOCATION ON CHEMICAL COMPOSITION AND ANTI-INFLAMMATORYACTIVITY (IN VITRO) OF D. HISPIDA TUBERS
Date
2023
Authors
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Publisher
International Medical University
Abstract
Dioscorea hispida Dennst., also known as "ubi gadong" in Malaysia, is a unique
species of climbing yam found in several Asian nations and known for its medicinal
properties despite its inherent toxicity due to the presence of the dioscorine compound.
This study, a collaboration between the Herbal Medicine Research Centre (HMRC),
the Institute of Medical Research (IMR) of Malaysia, and the International Medical
University (IMU), aimed to investigate the chemical composition and antiinflammatory
potential of D. hispida tuber extracts from Manir, Terengganu, and Jeli,
Kelantan. Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas
Chromatography-Mass Spectrometry (GC-MS) were used to determine the chemical
composition, and High-Performance Thin-Layer Chromatography (HPTLC) was used
to identify the presence of marker compounds including dioscorine, allantoin,
diosgenin and dioscin. On RAW 264.7 and BV-2 cell lines, the anti-inflammatory
potential of the extracts was evaluated using the Griess assay. Using real-time
quantitative polymerase chain reaction (RT-qPCR) and Enzyme-Linked
Immunosorbent Assay (ELISA), respectively, the effects of the extracts on the
expression of interleukin-6 (IL-6) and interleukin-1beta (IL-1β) genes and proteins
have been investigated. All of the samples contained allantoin, diosgenin, dioscin, and
dioscorine. Additionally, there were significant differences in the chemical
composition and anti-inflammatory activity of the samples, which may be influenced
by their geographical locations. Manir and Jeli extracts exhibited significant antiinflammatory
properties, with the Manir extract exhibiting a significant reversal
upregulation of IL-6 and IL-1β than Jeli extracts indicating a significant different in
collecting sites to anti-inflammatory effect. These findings highlight the importance of considering geographical variations in the study of herbal products, as they have a
direct impact on the effectiveness of the bioactive compounds that are produced.
Keywords: Dioscorea hispida, Extraction, Anti-inflammatory, Chemical composition
Description
Keywords
Dioscorea, Inflammation, Liquid Chromatography-Mass Spectrometry, Gas Chromatography-Mass Spectrometry, Polymerase Chain Reaction, Herbal