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THE INFLUENCE OF GEOGRAPHICAL LOCATION ON CHEMICAL COMPOSITION AND ANTI-INFLAMMATORYACTIVITY (IN VITRO) OF D. HISPIDA TUBERS

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dc.contributor.authorCHIA XIAO JUN
dc.date.accessioned2024-03-07T05:31:14Z
dc.date.available2024-03-07T05:31:14Z
dc.date.issued2023
dc.description.abstractDioscorea hispida Dennst., also known as "ubi gadong" in Malaysia, is a unique species of climbing yam found in several Asian nations and known for its medicinal properties despite its inherent toxicity due to the presence of the dioscorine compound. This study, a collaboration between the Herbal Medicine Research Centre (HMRC), the Institute of Medical Research (IMR) of Malaysia, and the International Medical University (IMU), aimed to investigate the chemical composition and antiinflammatory potential of D. hispida tuber extracts from Manir, Terengganu, and Jeli, Kelantan. Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) were used to determine the chemical composition, and High-Performance Thin-Layer Chromatography (HPTLC) was used to identify the presence of marker compounds including dioscorine, allantoin, diosgenin and dioscin. On RAW 264.7 and BV-2 cell lines, the anti-inflammatory potential of the extracts was evaluated using the Griess assay. Using real-time quantitative polymerase chain reaction (RT-qPCR) and Enzyme-Linked Immunosorbent Assay (ELISA), respectively, the effects of the extracts on the expression of interleukin-6 (IL-6) and interleukin-1beta (IL-1β) genes and proteins have been investigated. All of the samples contained allantoin, diosgenin, dioscin, and dioscorine. Additionally, there were significant differences in the chemical composition and anti-inflammatory activity of the samples, which may be influenced by their geographical locations. Manir and Jeli extracts exhibited significant antiinflammatory properties, with the Manir extract exhibiting a significant reversal upregulation of IL-6 and IL-1β than Jeli extracts indicating a significant different in collecting sites to anti-inflammatory effect. These findings highlight the importance of considering geographical variations in the study of herbal products, as they have a direct impact on the effectiveness of the bioactive compounds that are produced. Keywords: Dioscorea hispida, Extraction, Anti-inflammatory, Chemical composition
dc.identifier.urihttps://hdl.handle.net/20.500.14377/36050
dc.language.isoen
dc.publisherInternational Medical University
dc.subjectDioscorea
dc.subjectInflammation
dc.subjectLiquid Chromatography-Mass Spectrometry
dc.subjectGas Chromatography-Mass Spectrometry
dc.subjectPolymerase Chain Reaction
dc.subjectHerbal
dc.titleTHE INFLUENCE OF GEOGRAPHICAL LOCATION ON CHEMICAL COMPOSITION AND ANTI-INFLAMMATORYACTIVITY (IN VITRO) OF D. HISPIDA TUBERS
dc.typeThesis
dspace.entity.typePublication
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
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Theses (MSc. Analytical & Pharmaceutical Chemistry)